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The fresher something is, the more vitamins it has. vitamins dissappate because of contact with air, heat, and other reasons. That is why it is so much better to eat fruits right off the vine instead of out of a can.
But freezing holds in a lot more vitamins than cooking or canning (in the case of people food)...
So technically a fresh-killed mouse has more nutrients than f/t, but I still feed f/t because of cost, no chance of injury or parasites, and I don't want my snake to get "spoiled" and not go back to f/t after eating live.
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