Why not go back to the VERY beginning of the Sunkissed and/or SG lineage in considering how far this SG gene might have been spread? First off, the SKs came from Kathy Love and were being sold as Hypo Okeetees, I believe in the early to mid 90s. From what I recollect, Kathy said this line came spontaneously from a line of Okeetees she was working with. Does anyone SERIOUSLY believe that the SK and the SG genetic traits BOTH spontaneously showed up as mutations within a single animal? Especially since it has been shown that SG is NOT tied to SK?
When I bought my pair of SKs from Kathy, they were to be used as a shortcut for my own Hypo Okeetee project. I had a bunch of het adults nearly ready to breed and figured this would multiply my chances of getting the results I was looking for. This proved to be a disappointment, as every one of my Okeetees het Hypo that I bred those animals to produced nothing but normal colored animals, with NO hypomelanism evident. How many of those animals labelled as "Okeetees" have found their way into the other peoples' breeding stock? How many Okeetees sold by Kathy from this same line that originated the SK and SG genes are floating around in collections? Heck, a couple of years after I got those "Hypo Okeetees" from Kathy, she asked me about them concerning the breeding I had done with them. When I told her that I was outcrossing them and never bred them together, she told me she was relieved to hear that because she noted problems with the strain when breeding them together. I believe that is the first inkling of this SG issue that I can recall, but I really didn't inquire about what exactly those "problems" were that Kathy mentioned.
Based on what I am hearing, likely one of the two Sunkisseds I bought from Kathy was carrying the SG gene. They were used in outcrossing to all of the other genetic lines I worked with to produce combinations with this new genetic trait. And even then, I was working with parallel groups of genetic stock so as to limit inbreeding as much as was possible to do. I would breed the male to a female, say Lavender, and the female with a male Lavender with the intention of breeding the two lines back together again instead of breeding direct siblings together from a single clutch. So you can see how if just one of the two were a carrier how my breeding regimen would have greatly reduced the chances of any deleterious gene showing up in later generations. I ALWAYS outcrossed as much as was possible.
My guess is that it would have had to be the female Sunkissed that was carrying the SG gene, just because of the low frequency of this trait actually showing up in later years, and the simple fact that the male was used MUCH more than the female in breedings. Which meant the male was the ancestor of a lot more animals than the female was. Personally, out of the thousands of animals I hatched out every year, I only saw maybe a half dozen animals that could have potentially been SGs, and that was not until the last couple of years before I retired. But the numbers were no more statistically significant than the number of kinked animals I got from Lavender related animals, and other abnormalities that will be seen in any large sampling of babies hatching out. And this SG trait is so similar to other abnormalities in baby corn snakes that can be caused by environmental factors, that it would have been impractical and premature to make a positive statement either way about it. Heck, I hatched out animals in the mid 80s from a line I was calling my "Milk Snake Phase" Miamis that just acted strange in that they didn't care whether they were right side up or upside down. Connie called them the "Pineapple Upside Down Snakes". I actually kept a pair and bred them together and the trait did not surface in their offspring. So what caused it? Who knows? Anyone who breeds substantial numbers of baby snakes is going to see all sorts of questionable and sometimes heartbreaking abnormalities. It's just the percentages at work and the nature of what we are doing. If you have one in a thousand chances of getting a problem show up, and you hatch out 6,000 babies, well, statistically you will get six (6) problems hatch out of that batch. Some years you will beat the odds, and some years the odds will beat you. You know it's going to happen, so you just make the best of it and move on.
So no, I did not KNOW I had SG in my stock from that original female animal I got from Kathy. I STILL don't know, and am merely speculating based on evidence only recently being presented. And quite honestly, since I was outcrossing everything, I really was not all that concerned about it being a possibility when it first started becoming common knowledge. At that time (nearly a decade after I started working with them) there was not even semi-concrete evidence that either of the two Sunkisseds I originally got carried SG. Which is why I looked askance at Joe's claim that EVERY line of SK is "50% possible het SG". That implies that HALF of all SK animals that exist today WILL be het for SG, which, quite frankly, is just a completely ridiculous statement for someone to make. The more you outcross any animal with any gene, the LESS the probability is that it will show up. I possibly had one original Sunkissed from Kathy het for SG in my collection and because I outcrossed the pair, the gene was so heavily diluted in my collection that it turned out to be extremely rare to have even suspicious animals manifest such a trait. If EVERY Sunkissed (and related progeny) were a "50% PH" then I would have been up to my eyeballs in them every year when the babies hatched.
As for testing ONLY Sunkisseds for SG, seriously, if you are that concerned with getting the gene in your collection, then you need to test ALL genetic lines or simply get out of corn snakes completely. Kathy's Okeetees that she sold potentially carried that gene. Did she cross those "Hypo Okeetees" with anything else? Were the Okeetees themselves that were the founder stock of the SG gene outcrossed into anything else? Did anyone getting Okeetees from Kathy outcross THEM into anything else? SG and SK are two separate genes remember? The odds that SG was ONLY in the original SKs and STAYED within that line in the original founder stock would have to be EXTREMELY slim.
As for myself, I outcrossed the pair of Sunkisseds I got from Kathy with EVERY genetic strain I had at my facility (I doubt that anyone else would have done any differently getting a new genetic line in their hands) and certainly did NOT keep all of the offspring. Included in the culling were those full homozygous animals coming from the crosses that were not the combinations I was shooting for with Sunkissed. Not to mention thousands of normal colored, amelanistic, anerythristic, and hypomelanistic culls that went to wholesale brokers for resale over the years since I originally got that pair of Sunkisseds.
Nearly everyone claims that inbreeding is not a good idea for the corn snakes you work with anyway, so for crying out loud, just OUTCROSS them, and reduce the odds of ANY deleterious gene popping up. USE the odds to your advantage whenever you can.